A fungus engineered to produce a spider toxin could help take down insecticide-resistant mosquitoes that can spread malaria.
In a netted, outdoor experiment in Burkina Faso, the genetically engineered fungus wiped out mosquito populations within two generations, researchers report in the May 31 Science. If the result holds up in a real-world situation, the modified fungus may one day become a tool for controlling mosquitoes that can transmit the deadly disease.
In 2017, an estimated 219 million people in 87 countries were infected with malaria, and 435,000 died, according to the World Health Organization. Africa carried most of the malaria burden, with 92 percent of cases and 93 percent of deaths occurring on the continent that year.
The fungus Metarhizium pingshaense, long known to infect and kill mosquitoes, was made even deadlier to the insects by the addition of a gene that produces a spider bite toxin called Hybrid. Researchers engineered the fungus to make Hybrid in the presence of the mosquito version of blood, called hemolymph. “Were just bypassing the spider fangs and getting the fungus to do the same job,” says study coauthor Raymond St. Leger, an entomologist at the University of Maryland in College Park.
In laboratory trials in 2011, engineered fungi related to M. pingshaense infected and killed mosquitoes and their malaria parasites (SN Online: 2/25/11). (The fungi dont harm people, other insects or animals.) “Thats all well and good, but what happens in the lab doesnt necessarily translate into field conditions,” says study coauthor Brian Lovett, an insect pathologist and bioengineer also at the University of Maryland.
The fungi dont hold up well in heat or under ultraviolet light and needed to be tested under real-world conditions. But because the engineered M. pingshaense carries a foreign gene, “we cant just waltz out into the open field and start applying it to peoples houses,” Lovett says.
Working with scientists and villagers in a western region of Burkina Faso where malaria is endemic, the researchers built a giant frame enclosed by two layers of mosquito netting that was divided into sections with huts on the inside. In each hut, a black cloth coated in sesame oil hung on one wall, providing a place for female mosquitoes to rest after feeding. The oil helped fungal spores stick to the cloth. Huts contained a cloth with either no spores, normal spores that dont make the spider toxin or Hybrid-producing spores.
Each hut was then populated with 1,000 adult male mosquitoes and 500 females, grown from larvae and eggs from insecticide-resistant Anopheles coluzzii collected by local people from puddles. In mating, females dive into a swarm of males; after mating, females must feed on blood to support egg-laying. The mosquitoes drank blood from calves introduced into the huts for three nights each week. The researchers then counted how many adult mosquitoes survived in subsequent generations.
A genetically engineered version of the fungus Metarhizium pingshaense (orange line) killed more mosquitoes more quickly than the unaltered fungus (yellow) in a net-enclosed outdoor study. Both versions of the fungus killed roughly 75 percent of mosquitoes over two weeks, but the engineered fungus worked faster. Control groups of mosquitoes were exposed to neither fungus.
Survival of captured mosquitoes following exposure to a fungus engineered to make a spider toxin
In the hut without the fungus, 921 mosquitoes hatched in the first generation and 1,396 in the second about 25 days later. In the hut with the nonengineered fungus, fewer mosquitoes hatched, with just 436 mosquitoes in the first generation and 455 in the second. Thats a sign that the fungus alone kept numbers down, but didnt eliminate the insects.
In the hut with the toxin-producing fungus, 399 mosquitoes hatched in the first generation. But in the second generatioRead More – Source